Next-Generation Sequencing (NGS) significantly accelerates drug discovery and development by providing comprehensive insights into the genetic determinants of diseases. It enables the identification of novel targets by revealing mutations, gene expressions, and complex biomarkers associated with various conditions. This detailed genomic understanding helps in designing targeted therapies that are more effective and have fewer side effects. Furthermore, NGS facilitates patient stratification in clinical trials, ensuring that treatments are tested on populations most likely to benefit, thereby increasing the efficiency of trials and the speed at which new drugs can be brought to market. Overall, NGS empowers the development of personalized medicine, allowing for treatments that are tailored to the genetic makeup of individual patients, enhancing therapeutic outcomes and reducing the burden of adverse reactions.