The application of Next-Generation Sequencing (NGS) in pharmacogenomics has been transformative, enabling a more nuanced understanding of how genetic variations affect individual responses to medications. By sequencing a patient's genome, NGS allows for the identification of specific genetic markers that influence drug metabolism, efficacy, and risk of adverse reactions. This personalized approach to medicine not only improves patient outcomes by guiding the selection of optimal drug therapies and dosages for individual patients but also aids in the development of new drugs by identifying target genetic profiles for treatment. Consequently, NGS in pharmacogenomics is paving the way toward a future where healthcare is tailored to the genetic makeup of each patient, minimizing trial and error in prescribing medications and enhancing the safety and effectiveness of treatments.